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Targeting to Fcgamma receptors, but not CR3 (CD11b/CD18), increases clearance of Bordetella pertussis.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 2001 Mar 15; Vol. 183 (6), pp. 871-9. Date of Electronic Publication: 2001 Feb 13. - Publication Year :
- 2001
-
Abstract
- In the absence of opsonizing antibodies, Bordetella pertussis, the causative agent of pertussis, readily binds to phagocytes via complement receptor 3 (CR3). After opsonization with antibodies, binding is mediated by IgG receptors (FcgammaR). The effect of targeting B. pertussis to either FcgammaR or CR3 was studied. The fate of unopsonized B. pertussis, IgG-opsonized B. pertussis, and B. pertussis opsonized with bispecific antibodies (BsAbs) directed to CR3 or FcgammaRII/-III was compared. IgG antibodies mediated binding and phagocytosis of B. pertussis via FcgammaR by polymorphonuclear leukocytes (PMNL) in vitro. Opsonization of B. pertussis with BsAbs directed against either CR3 or FcgammaRII/-III facilitated PMNL phagocytosis; however, in vivo studies with BsAb revealed that FcgammaR-mediated uptake facilitates B. pertussis clearance, in contrast to uptake via CR3. Targeting of B. pertussis to FcgammaRII/-III in mice deficient in FcgammaRII or FcgammaRIII indicated that the protective effect is attributable to FcgammaRIII. Competition between uptake via CR3 or FcgammaR may determine the outcome of natural infection.
- Subjects :
- Animals
Antibodies, Bispecific immunology
Cells, Cultured
Female
Immunoglobulin G immunology
Macrophage-1 Antigen genetics
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Neutrophils immunology
Opsonin Proteins immunology
Receptors, IgG genetics
Whooping Cough immunology
Antibodies, Bacterial immunology
Bordetella pertussis immunology
Macrophage-1 Antigen immunology
Phagocytosis
Receptors, IgG immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1899
- Volume :
- 183
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 11237803
- Full Text :
- https://doi.org/10.1086/319266