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The physical maps for sequencing human chromosomes 1, 6, 9, 10, 13, 20 and X.

Authors :
Bentley DR
Deloukas P
Dunham A
French L
Gregory SG
Humphray SJ
Mungall AJ
Ross MT
Carter NP
Dunham I
Scott CE
Ashcroft KJ
Atkinson AL
Aubin K
Beare DM
Bethel G
Brady N
Brook JC
Burford DC
Burrill WD
Burrows C
Butler AP
Carder C
Catanese JJ
Clee CM
Clegg SM
Cobley V
Coffey AJ
Cole CG
Collins JE
Conquer JS
Cooper RA
Culley KM
Dawson E
Dearden FL
Durbin RM
de Jong PJ
Dhami PD
Earthrowl ME
Edwards CA
Evans RS
Gillson CJ
Ghori J
Green L
Gwilliam R
Halls KS
Hammond S
Harper GL
Heathcott RW
Holden JL
Holloway E
Hopkins BL
Howard PJ
Howell GR
Huckle EJ
Hughes J
Hunt PJ
Hunt SE
Izmajlowicz M
Jones CA
Joseph SS
Laird G
Langford CF
Lehvaslaiho MH
Leversha MA
McCann OT
McDonald LM
McDowall J
Maslen GL
Mistry D
Moschonas NK
Neocleous V
Pearson DM
Phillips KJ
Porter KM
Prathalingam SR
Ramsey YH
Ranby SA
Rice CM
Rogers J
Rogers LJ
Sarafidou T
Scott DJ
Sharp GJ
Shaw-Smith CJ
Smink LJ
Soderlund C
Sotheran EC
Steingruber HE
Sulston JE
Taylor A
Taylor RG
Thorpe AA
Tinsley E
Warry GL
Whittaker A
Whittaker P
Williams SH
Wilmer TE
Wooster R
Wright CL
Source :
Nature [Nature] 2001 Feb 15; Vol. 409 (6822), pp. 942-3.
Publication Year :
2001

Abstract

We constructed maps for eight chromosomes (1, 6, 9, 10, 13, 20, X and (previously) 22), representing one-third of the genome, by building landmark maps, isolating bacterial clones and assembling contigs. By this approach, we could establish the long-range organization of the maps early in the project, and all contig extension, gap closure and problem-solving was simplified by containment within local regions. The maps currently represent more than 94% of the euchromatic (gene-containing) regions of these chromosomes in 176 contigs, and contain 96% of the chromosome-specific markers in the human gene map. By measuring the remaining gaps, we can assess chromosome length and coverage in sequenced clones.

Details

Language :
English
ISSN :
0028-0836
Volume :
409
Issue :
6822
Database :
MEDLINE
Journal :
Nature
Publication Type :
Academic Journal
Accession number :
11237015
Full Text :
https://doi.org/10.1038/35057165