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Identification of a contaminating Mycobacterium tuberculosis strain with a transposition of an IS6110 insertion element resulting in an altered spoligotype.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 2001 Mar; Vol. 39 (3), pp. 1092-6. - Publication Year :
- 2001
-
Abstract
- Molecular fingerprinting with the IS6110 insertion sequence is useful for tracking transmission of Mycobacterium tuberculosis within a population or confirming specimen contamination in the laboratory or through instrumentation. Secondary typing with other molecular methods yields additional information as to the relatedness of strains with similar IS6110 fingerprints. Isolated, relatively rare, random events within the M. tuberculosis genome alter molecular fingerprinting patterns with any of the methods; therefore, strains which are different by two or more typing methods are usually not considered to be closely related. In this report, we describe two strains of M. tuberculosis, obtained from the same bronchoscope 2 days apart, that demonstrated unique molecular fingerprinting patterns by two different typing methods. They were closely linked through the bronchoscope by a traditional epidemiologic investigation. Genetic analysis of the two strains revealed that a single event, the transposition of an IS6110 insertion sequence in one of the strains, accounted for both the differences in the IS6110 pattern and the apparent deletion of a spacer in the spoligotype. This finding shows that a single event can change the molecular fingerprint of a strain in two different molecular typing systems, and thus, molecular typing cannot be the only means used to track transmission of this organism through a population. Traditional epidemiologic techniques are a necessary complement to molecular fingerprinting so that radical changes within the fingerprint pattern can be identified.
- Subjects :
- Bacterial Typing Techniques
Base Sequence
DNA, Intergenic genetics
Humans
Male
Middle Aged
Molecular Sequence Data
Oligonucleotides analysis
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Repetitive Sequences, Nucleic Acid genetics
Tuberculosis, Pulmonary microbiology
Bronchoscopes
DNA Transposable Elements
Equipment Contamination
Mycobacterium tuberculosis classification
Mycobacterium tuberculosis genetics
Tuberculosis, Pulmonary epidemiology
Subjects
Details
- Language :
- English
- ISSN :
- 0095-1137
- Volume :
- 39
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 11230432
- Full Text :
- https://doi.org/10.1128/JCM.37.3.1092-1096.2001