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Spatial arrangements and associative behavior of species in an in vitro oral biofilm model.
- Source :
-
Applied and environmental microbiology [Appl Environ Microbiol] 2001 Mar; Vol. 67 (3), pp. 1343-50. - Publication Year :
- 2001
-
Abstract
- The spatial arrangements and associative behavior of Actinomyces naeslundii, Veillonella dispar, Fusobacterium nucleatum, Streptococcus sobrinus, and Streptococcus oralis strains in an in vitro model of supragingival plaque were determined. Using species-specific fluorescence-labeled antibodies in conjunction with confocal laser scanning microscopy, the volumes and distribution of the five strains were assessed during biofilm formation. The volume-derived cell numbers of each strain correlated well with respective culture data. Between 15 min and 64 h, populations of each strain increased in a manner reminiscent of batch growth. The microcolony morphologies of all members of the consortium and their distributions within the biofilm were characterized, as were interspecies associations. Biofilms formed 15 min after inoculation consisted principally of single nonaggregated cells. All five strains adhered strongly to the saliva-conditioned substratum, and therefore, coadhesion played no role during the initial phase of biofilm formation. This observation does not reflect the results of in vitro coaggregation of the five strains, which depended upon the nature of the suspension medium. While the possibility cannot be excluded that some interspecies associations observed at later stages of biofilm formation were initiated by coadhesion, increase in bacterial numbers appeared to be largely a growth phenomenon regulated by the prevailing cultivation conditions.
- Subjects :
- Actinomyces growth & development
Antibodies, Monoclonal immunology
Biofilms growth & development
Culture Media
Fusobacterium growth & development
Humans
Microscopy, Confocal
Species Specificity
Streptococcus oralis growth & development
Streptococcus sobrinus growth & development
Veillonella growth & development
Dental Plaque microbiology
Gingiva microbiology
Models, Biological
Subjects
Details
- Language :
- English
- ISSN :
- 0099-2240
- Volume :
- 67
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Applied and environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 11229930
- Full Text :
- https://doi.org/10.1128/AEM.67.3.1343-1350.2001