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Isolation of a Candida albicans gene, tightly linked to URA3, coding for a putative transcription factor that suppresses a Saccharomyces cerevisiae aft1 mutation.
- Source :
-
Yeast (Chichester, England) [Yeast] 2001 Mar 15; Vol. 18 (4), pp. 301-11. - Publication Year :
- 2001
-
Abstract
- A pathogen such as C. albicans needs an efficient mechanism of iron uptake in an iron-restricted environment such as is the human body. A ferric-reductase activity regulated by iron and copper, and analogous to that in S. cerevisiae, has been described in C. albicans. We have developed an in-plate protocol for the isolation of clones that complement an aft1 mutation in S. cerevisiae that makes cells dependent on iron for growth. After transformation of S. cerevisiae aft1 with a C. albicans library, we have selected clones that grow in conditions of iron deficiency and share an identical plasmid, pIRO1, with a 4500 bp insert containing the URA3 gene and an ORF (IRO1) responsible for the suppression of the iron dependency. IRO1 does not show homology with AFT1 or with other sequences in the databases. Northern analysis demonstrates constitutive expression of IRO1. CAI4, a C. albicans strain isolated as Deltaura3, also has a deletion of the 3' half of IRO1, and displays in YNB medium similar phenotypic characteristics to S. cerevisiae aft1 mutant strains. Therefore, we consider IRO1 as a gene of C. albicans involved in the utilization of iron. However, in extreme conditions of iron deprivation, CAI4 seems to activate alternative mechanisms of iron uptake that allow a better growth than the wild strain SC5314. Analysis of its predicted protein sequence is in agreement with a role of Iro1p as a transcription factor.<br /> (Copyright 2000 John Wiley & Sons, Ltd.)
- Subjects :
- Amino Acid Sequence
Candida albicans cytology
Candida albicans metabolism
Genetic Linkage
Molecular Sequence Data
Orotidine-5'-Phosphate Decarboxylase genetics
Saccharomyces cerevisiae genetics
Sequence Homology, Amino Acid
Suppression, Genetic
Candida albicans genetics
Fungal Proteins genetics
Genes, Fungal
Iron metabolism
Saccharomyces cerevisiae Proteins
Transcription Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0749-503X
- Volume :
- 18
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Yeast (Chichester, England)
- Publication Type :
- Academic Journal
- Accession number :
- 11223939
- Full Text :
- https://doi.org/10.1002/1097-0061(20010315)18:4<301::AID-YEA672>3.0.CO;2-H