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Oxygen radical generation by emigrated, intra-abdominal, and circulating PMNLs during human secondary peritonitis.
- Source :
-
Shock (Augusta, Ga.) [Shock] 2001 Feb; Vol. 15 (2), pp. 90-4. - Publication Year :
- 2001
-
Abstract
- The purpose of the study was to characterize oxygen radical generation by emigrated, intraabdominal and circulating polymorphonuclear leukocytes (ePMNLs and cPMNLs) during peritonitis, as well as to assess any differences between oxygen radical production in patients with low Mannheim peritonitis index (MPI < 26, group 1) or high Mannheim peritonitis index (MPI > or = 26, group 2). Lucigenin-enhanced chemiluminescence was used to determine spontaneous and stimulated (FMLP, PMA, and A23 187) oxygen radical generation by ePMNLs and cPMNLs. In group 1 spontaneous and stimulated oxygen radical generation by emigrated PMNLs was markedly enhanced compared to circulating PMNLs (e.g., spontaneous oxygen radical generation: 30.3 +/- 11.8 cpm/cPMNLs versus 107 +/- 46 cpm/ePMNLs, P < 0.05) . In group 2 oxygen radical generation by cPMNLs markedly increased within 48 h after diagnosis of peritonitis and surgery, contrary to radical generation by ePMNLs (e.g., A23 187-stimulated oxygen radical generation 993.7 +/- 350 cpm/cPMNLs versus 285.6 +/- 77 cpm/ePMNLs, P < 0.05. In conclusion, cPMNLs and ePMNLs exhibit marked polymorphism in their capacity to generate oxygen radicals in response to secondary peritonitis. Severe peritonitis (MPI - 26) was associated with a strong increase in oxygen radical generation by cPMNLs without a parallel activity being manifest by ePMNLs.
- Subjects :
- Abdomen surgery
Adult
Aged
Case-Control Studies
Cell Survival
Endotoxins blood
Exudates and Transudates metabolism
Female
Free Radicals
Humans
Interleukin-8 blood
Male
Middle Aged
Peritonitis mortality
Peritonitis surgery
Prognosis
Tumor Necrosis Factor-alpha metabolism
Neutrophils metabolism
Oxygen metabolism
Peritonitis blood
Subjects
Details
- Language :
- English
- ISSN :
- 1073-2322
- Volume :
- 15
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Shock (Augusta, Ga.)
- Publication Type :
- Academic Journal
- Accession number :
- 11220647
- Full Text :
- https://doi.org/10.1097/00024382-200115020-00002