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Real-time detection of insulin-like growth factor-1 stimulation of the MAC-T bovine mammary epithelial cell line.
- Source :
-
Endocrine [Endocrine] 2000 Dec; Vol. 13 (3), pp. 345-52. - Publication Year :
- 2000
-
Abstract
- Binding of growth factors by cell-surface receptors is an essential means by which cells regulate normal tissue growth and differentiation. Exposure to growth factors is often transient, and our goal was to determine whether short-term exposure to insulin-like growth factor-1 (IGF-1) would lead to activation, assayed as cell proliferation, of mammary epithelial cells. The MAC-T cell line is an immortalized bovine mammary epithelial cell line, chosen as our model mammary cell line because of its known sensitivity to IGF-1. Using the Cytosensor Microphysiometer System, a biosensor capable of measuring extracellular acidification, we were able to measure activation of the cells owing to IGF-1 addition in real time and found that peak acidification occurred in only 14 min. We show that this rapid response to IGF-1 is dose dependent and specific for IGF-1. A significant increase in [3H]thymidine incorporation by cells after a similar short-term exposure to IGF-1 suggests that the measured increase in extracellular acidification following IGF-1 addition is physiologically relevant. This technology offers a new, novel, and rapid means for the study of IGF-1 activity, as well as the screening of IGF-1 inhibitors, in mammary epithelial cells.
- Subjects :
- Animals
Cattle
Cell Count
Cell Division
Cell Line, Transformed
DNA biosynthesis
Dose-Response Relationship, Drug
Epithelial Cells cytology
Epithelial Cells metabolism
Female
Hydrogen-Ion Concentration
Insulin pharmacology
Insulin-Like Growth Factor I administration & dosage
Kinetics
Mammary Glands, Animal metabolism
Thymidine metabolism
Tritium
Insulin-Like Growth Factor I pharmacology
Mammary Glands, Animal cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1355-008X
- Volume :
- 13
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Endocrine
- Publication Type :
- Academic Journal
- Accession number :
- 11216647
- Full Text :
- https://doi.org/10.1385/ENDO:13:3:345