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Carrier protein-modulated presentation and recognition of an N-glycan: observations on the interactions of Man(8) glycoform of ribonuclease B with conglutinin.
- Source :
-
Glycobiology [Glycobiology] 2001 Jan; Vol. 11 (1), pp. 31-6. - Publication Year :
- 2001
-
Abstract
- Conglutinin is a serum lectin of the innate immune system, which binds high mannose N-glycans when these are appropriately presented on proteins. Here we use the conglutinin-ribonuclease B (RNaseB)-recognition system as a model to investigate the structural basis of selective recognition of protein-bound oligosaccharides by this carbohydrate-binding receptor. Conglutinin shows little binding to the isolated RNaseB-Man(8 )glycoform, and no binding to Man(5-6) glycoforms. In contrast, when the protein moiety is reduced and denatured we observe that conglutinin binds strongly to the isolated RNaseB-Man(8) glycoform and weakly to the Man(5-6) glycoforms. These results are in accord with observations on the binding to the N-glycans in the absence of carrier protein. NMR analyses of native RNaseB-Man(8) and -Man(5-6) glycoforms reveal that the three-dimensional structure of the protein moiety is essentially identical to that of non-glycosylated RNase (RNaseA). Thus there are no perceptible differences between the RNase protein forms that could account for differential availability of the N-glycan for conglutinin-binding. After reduction and denaturation, the NMR spectrum became typical of a non-structured polypeptide, although the conformational preferences of the N-glycosidic linkage were unchanged, and most importantly, the Man(8 )oligosaccharide retained the average conformational behavior of the free oligosaccharide irrespective of the carrier protein fold. This conformational freedom is clearly not translated into full availability of the oligosaccharide for the carbohydrate-recognition protein. We propose, therefore, that the differing bioactivity of the N-glycan is a reflection of the existence of different geometries of presentation of the carbohydrate determinant in relation to the protein surface within the glycan:carrier protein ensemble.
- Subjects :
- Carbohydrate Conformation
Carbohydrate Sequence
Mannose chemistry
Molecular Sequence Data
Nuclear Magnetic Resonance, Biomolecular
Oxidation-Reduction
Protein Binding
Protein Denaturation
Ribonucleases chemistry
Carrier Proteins metabolism
Collectins
Mannose metabolism
Polysaccharides metabolism
Ribonucleases metabolism
Serum Globulins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0959-6658
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Glycobiology
- Publication Type :
- Academic Journal
- Accession number :
- 11181559
- Full Text :
- https://doi.org/10.1093/glycob/11.1.31