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Membrane topology and immunolocalization of CHIF in kidney and intestine.
- Source :
-
American journal of physiology. Renal physiology [Am J Physiol Renal Physiol] 2001 Mar; Vol. 280 (3), pp. F505-12. - Publication Year :
- 2001
-
Abstract
- Corticosteroid hormone-induced factor (CHIF) is an aldosterone-induced gene, the function of which is yet unknown. It is specifically expressed in kidney collecting duct (CD) and distal colon and is upregulated by either Na+ deprivation or K+ loading. Hence, it may play a role in epithelial electrolyte transport. Previous studies have characterized regulation and tissue distribution of CHIF mRNA but provided no information on the protein itself. The present paper addresses this issue by using Western blotting, immunochemistry, and in vitro translation. CHIF is an approximately 8-kDa membranal protein, and protease digestion experiments suggest that its COOH tail faces the cell interior. The protein is abundant in distal colon, kidney medulla, and papilla but cannot be detected in a variety of other tissues. Confocal immunocytochemistry demonstrates that CHIF is present in the basolateral membrane of CD principal cells and distal colon surface cells, with occasional intracellular staining. Dexamethasone and low Na+ intake increase the abundance of CHIF. Unlike previous Northern data, induction of CHIF protein by low-Na+ intake was apparent not only in the distal colon but also in the kidney.
- Subjects :
- Animals
Blotting, Western
Cell Line
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Kidney Tubules, Collecting cytology
Kidney Tubules, Collecting metabolism
Male
Membrane Proteins genetics
Membrane Proteins metabolism
RNA, Messenger metabolism
Rats
Rats, Sprague-Dawley
Rats, Wistar
Reticulocytes metabolism
Tissue Distribution
Colon metabolism
Intracellular Membranes metabolism
Kidney metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1931-857X
- Volume :
- 280
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Renal physiology
- Publication Type :
- Academic Journal
- Accession number :
- 11181413
- Full Text :
- https://doi.org/10.1152/ajprenal.2001.280.3.F505