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Presence of soluble and particulate guanylyl cyclase in the same hippocampal astrocytes.
- Source :
-
Brain research [Brain Res] 2001 Feb 09; Vol. 891 (1-2), pp. 206-12. - Publication Year :
- 2001
-
Abstract
- The localisation of particulate and soluble guanylyl cyclase was studied in hippocampal astrocytes. Counting the colocalisation of cGMP immunoreactivity with the astrocytic marker glial fibrillary acidic protein after stimulation of brain slices with sodium nitroprusside (0.1 mM) or atrial natriuretic peptide (100 nM), we were able to show that at least 67% of the hippocampal astrocytes contained both guanylyl cyclase isoforms. In addition, it was shown that a large number of atrial natriuretic peptide, brain-derived natriuretic peptide or sodium nitroprusside responsive cells contain the beta1-subunit of the soluble guanylyl cyclase. The results show that, in at least a subset of hippocampal astrocytes, soluble and particulate guanylyl cyclases are simultaneously present in the same cells.
- Subjects :
- 1-Methyl-3-isobutylxanthine pharmacology
Animals
Astrocytes cytology
Astrocytes drug effects
Atrial Natriuretic Factor metabolism
Atrial Natriuretic Factor pharmacology
Cyclic GMP biosynthesis
Glial Fibrillary Acidic Protein metabolism
Hippocampus cytology
Hippocampus drug effects
Isoenzymes metabolism
Male
Nitroprusside pharmacology
Phosphodiesterase Inhibitors pharmacology
Rats
Rats, Inbred Lew
Solubility
Vasodilator Agents pharmacology
Astrocytes enzymology
Guanylate Cyclase metabolism
Hippocampus enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-8993
- Volume :
- 891
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Brain research
- Publication Type :
- Academic Journal
- Accession number :
- 11164824
- Full Text :
- https://doi.org/10.1016/s0006-8993(00)03213-3