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Synthesis and in vitro evaluation of 123I-labelled human recombinant annexin V.
- Source :
-
Biomolecular engineering [Biomol Eng] 2001 Jan; Vol. 17 (2), pp. 51-3. - Publication Year :
- 2001
-
Abstract
- Annexin V was radiolabelled with iodine-123 in order to develop a SPECT-ligand for imaging atherosclerosis and apoptosis. Iodination by means of electrophylic substitution resulted in radiochemical yields up to 70% and specific activities of 7.4-92.5 MBq/microg protein. Binding experiments with blood platelets indicated that 123I-labelled annexin V remained its biological activity.
- Subjects :
- Blood Platelets metabolism
Chromatography, High Pressure Liquid
Dose-Response Relationship, Drug
Humans
Iodine Radioisotopes chemistry
Ligands
Protein Binding
Time Factors
Tomography, Emission-Computed, Single-Photon methods
Annexin A5 chemical synthesis
Recombinant Proteins chemical synthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1389-0344
- Volume :
- 17
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biomolecular engineering
- Publication Type :
- Academic Journal
- Accession number :
- 11163751
- Full Text :
- https://doi.org/10.1016/s1389-0344(00)00062-9