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Geranylgeranylacetone induces antiviral gene expression in human hepatoma cells.

Authors :
Ichikawa T
Nakao K
Nakata K
Hamasaki K
Takeda Y
Kajiya Y
Higashi S
Ohkubo K
Kato Y
Ishii N
Eguchi K
Source :
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2001 Jan 26; Vol. 280 (3), pp. 933-9.
Publication Year :
2001

Abstract

Geranylgeranylacetone (GGA), an isoprenoid compound, is used clinically as an anti-ulcer drug. Since some isoprenoids including retinoids have anti-tumor and anti-viral activities in a variety of cell types, we investigated whether GGA could induce anti-viral proteins in human hepatoma cells. The HuH-7 and HepG2 cells were treated with GGA, and expression of anti-viral proteins such as 2'5'-oligoadenylate synthetase (2'5'-OAS) and double-stranded RNA-dependent protein kinase (PKR) in these cells was analyzed. GGA stimulated 2'5'-OAS and PKR gene expression at the transcriptional level through the formation of interferon-stimulated gene factor 3 (ISGF3), which regulates both gene transcription. By Western blotting, GGA induced expression of signal transducers and activators of transcription 1, 2 (STAT1, STAT2) and p48 proteins, components of ISGF3, together with the phosphorylation of STAT1. These results suggest that GGA acts as a potent inducer of anti-viral gene expression by stimulating the ISGF3 formation in human hepatoma cells.<br /> (Copyright 2001 Academic Press.)

Details

Language :
English
ISSN :
0006-291X
Volume :
280
Issue :
3
Database :
MEDLINE
Journal :
Biochemical and biophysical research communications
Publication Type :
Academic Journal
Accession number :
11162614
Full Text :
https://doi.org/10.1006/bbrc.2000.4228