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Synthetic 6B di-, tri-, and tetrasaccharide-protein conjugates contain pneumococcal type 6A and 6B common and 6B-specific epitopes that elicit protective antibodies in mice.
- Source :
-
Infection and immunity [Infect Immun] 2001 Feb; Vol. 69 (2), pp. 787-93. - Publication Year :
- 2001
-
Abstract
- The immunogenicity and protective capacity of Streptococcus pneumoniae 6B capsular polysaccharide (PS)-derived synthetic phosphate-containing disaccharide (Rha-ribitol-P-), trisaccharide (ribitol-P-Gal-Glc-), and tetrasaccharide (Rha-ribitol-P-Gal-Glc-)-protein conjugates in rabbits and mice were studied. In rabbits, all saccharides conjugated to keyhole limpet hemocyanin (KLH) evoked high levels of pneumococcal (Pn) type 6B antibodies that facilitated type-specific phagocytosis. Unlike the disaccharide rabbit antisera, tri- and tetrasaccharide rabbit antisera also reacted with 6A PS in an enzyme-linked immunosorbent assay (ELISA) and promoted phagocytosis of 6A pneumococci. All these rabbit antisera passively protected mice against a Pn 6B challenge. The disaccharide conjugate-induced antiserum, however, failed to protect mice against a 6A challenge. In mice, phagocytic and protective anti-Pn 6B antibodies were only induced by the tetrasaccharide conjugate and not by PS 6B or PS 6B-protein conjugates. These antibodies did not cross-react with 6A PS in ELISA and were unable to phagocytize 6A pneumococci. In conclusion, the disaccharide and tetrasaccharide conjugates already contain epitopes capable of inducing 6B-specific, fully protective antibodies in rabbits and mice, respectively.
- Subjects :
- Animals
Enzyme-Linked Immunosorbent Assay
Epitopes
Female
Hemocyanins immunology
Humans
Immune Sera immunology
Immunization, Passive
Mice
Mice, Inbred BALB C
Phagocytosis
Rabbits
Vaccines, Conjugate immunology
Antibodies, Bacterial biosynthesis
Oligosaccharides immunology
Pneumococcal Vaccines immunology
Streptococcus pneumoniae immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0019-9567
- Volume :
- 69
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 11159969
- Full Text :
- https://doi.org/10.1128/IAI.69.2.787-793.2001