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Discovery of two novel variants of KIR2DS5 reveals this gene to be a common component of human KIR 'B' haplotypes.
- Source :
-
Tissue antigens [Tissue Antigens] 2000 Nov; Vol. 56 (5), pp. 453-6. - Publication Year :
- 2000
-
Abstract
- The gene encoding the non-inhibitory receptor KIR2DS5 has so far been represented by a single cDNA sequence, NKAT9. A previous study by polymerase chain reaction using sequence-specific primers (PCR-SSP) failed to detect NKAT9 in genomic DNA of 52 donors, which suggested that KIR2DS5 could be a rare gene. Here, we have characterized two novel variants of KIR2DS5 that differ from NKAT9 by 8 and 10 nucleotide substitutions. The frequency of KIR2DS5 was then re-assessed by PCR-SSP using primers specific for conserved sequences of all three known KIR2DS5 variants. We found KIR2DS5 is not a rare gene, but one present in 26% of 34 donors representing the major ethnic groups. Like other non-inhibitory KIR, the distribution of KIR2DS5 is restricted to the 'B' subset of KIR-gene haplotypes. Transcription of the KIR2DS5 gene was studied by reverse transcriptase (RT)-PCR in natural killer (NK) cells from one donor and shown to follow the clonal distribution seen for most other KIR genes.
Details
- Language :
- English
- ISSN :
- 0001-2815
- Volume :
- 56
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Tissue antigens
- Publication Type :
- Academic Journal
- Accession number :
- 11144295
- Full Text :
- https://doi.org/10.1034/j.1399-0039.2000.560510.x