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Detection of T-2 toxin in different cereals by flow-through enzyme immunoassay with a simultaneous internal reference.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2000 Dec; Vol. 48 (12), pp. 5864-7. - Publication Year :
- 2000
-
Abstract
- In most previously described membrane-based immunoassays a separate negative control assay is always carried out to evaluate the performance of the assay. To overcome this problem, a membrane-based flow-through enzyme immunoassay with an internal control has been developed for the detection of T-2 toxin in cereals (patent pending). An Immunodyne ABC membrane was coated with 2 microL of goat anti-horseradish peroxidase (HRP) (internal control spot) (1:1000) and 2 microL of rabbit anti-mouse (test spot) (undiluted) immunoglobulins, and the free binding sites were blocked. In addition to the antibody-coated Immunodyne ABC membrane, the assay also comprises a plastic snap-fit device, absorbent cotton wool, mouse anti-T-2 monoclonal antibodies (Mab), and T-2-HRP conjugate. The color intensity (Delta) of the internal control and that of the negative sample showed no difference (P > 0.05), whereas there was a significant difference between the internal control and positive samples (P < 0.05). The minimum detectable limit that could be visually detected with confidence was 50 ng of T-2 per gram of cereal sample.
Details
- Language :
- English
- ISSN :
- 0021-8561
- Volume :
- 48
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 11141260
- Full Text :
- https://doi.org/10.1021/jf000337k