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An improved method for the detection of Norwalk-like caliciviruses in environmental samples.
- Source :
-
Letters in applied microbiology [Lett Appl Microbiol] 2000 Dec; Vol. 31 (6), pp. 411-5. - Publication Year :
- 2000
-
Abstract
- An original magnetic beads RNA capture was developed for the detection of Norwalk-like virus by RT-PCR. The same oligonucleotide was used both for capture and reverse transcription of the viral RNA. The optimization studies showed that the most important parameter for sensitivity is the biotin-binding capacity of the beads. This method was found to be efficient for eliminating inhibitors in sewage samples compared with the classic RT-PCR. Moreover, the sensitivity was greatly enhanced, allowing the detection of 42% positive sample after gel electrophoresis, which is fourfold greater than classic RT-PCR (11%). Beads-RT-PCR sensitivity is the same as classic RT-PCR and hybridization. Thus, this method, which is easy to perform, should be of particular interest for developing quantitative RT-PCR and sequencing.
Details
- Language :
- English
- ISSN :
- 0266-8254
- Volume :
- 31
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Letters in applied microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 11123547
- Full Text :
- https://doi.org/10.1046/j.1365-2672.2000.00837.x