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DLC-1 is deleted in primary hepatocellular carcinoma and exerts inhibitory effects on the proliferation of hepatoma cell lines with deleted DLC-1.
- Source :
-
Cancer research [Cancer Res] 2000 Dec 01; Vol. 60 (23), pp. 6581-4. - Publication Year :
- 2000
-
Abstract
- We investigated the expression and deletion of DLC-1 (frequently deleted in liver cancer gene), first reported in 1998 and having a high homology with rat p122RhoGAP in hepatocellular carcinoma (HCC). Six (20%) of 30 human HCC samples and 2 (40%) of 5 HCC cell lines were found to have no detectable DLC-1 expression by reverse transcription-PCR. Homozygous DLC-1 deletion was detected by Southern blotting in two of six HCC samples and in both HCC cell lines with no DLC-1 expression. Transfection of DLC-1 into 5 HCC cell lines (two with DLC-1 deletion and three with intact DLC-1) showed significant growth inhibition in these two HCC cell lines with deleted DLC-1 with both 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation assays but not in three other HCC cell lines with intact DLC-1. Our findings suggest that DLC-1 may play an important role in hepatocarcinogenesis.
- Subjects :
- Blotting, Southern
Carcinoma, Hepatocellular pathology
Cell Division genetics
GTPase-Activating Proteins
Gene Deletion
Gene Expression
Homozygote
Humans
Liver Neoplasms pathology
Protein Biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
Carcinoma, Hepatocellular genetics
Liver Neoplasms genetics
Proteins genetics
Tumor Suppressor Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0008-5472
- Volume :
- 60
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- Cancer research
- Publication Type :
- Academic Journal
- Accession number :
- 11118037