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Cloning and functional expression in Escherichia coli of a cDNA encoding cardenolide 16'-O-glucohydrolase from Digitalis lanata Ehrh.
- Source :
-
Plant & cell physiology [Plant Cell Physiol] 2000 Nov; Vol. 41 (11), pp. 1293-8. - Publication Year :
- 2000
-
Abstract
- A clone of cardenolide 16'-O-glucohydrolase cDNA (CGH I) was obtained from Digitalis lanata which encodes a protein of 642 amino acids (calculated molecular mass 73.2 kDa). The amino acid sequence derived from CGH I showed high homology to a widely distributed family of beta-glucohydrolases (glycosyl hydrolases family 1). The recombinant CGH I protein produced in Escherichia coli had CGH I activity. CGH I mRNA was detected in leaves, flowers, stems and fruits of D. lanata.
- Subjects :
- Amino Acid Sequence
Blotting, Northern
Cardenolides metabolism
Cloning, Molecular
DNA, Complementary chemistry
Digitalis enzymology
Gene Expression Regulation, Enzymologic
Glucosidases metabolism
Molecular Sequence Data
RNA, Messenger genetics
RNA, Messenger metabolism
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Tissue Distribution
DNA, Complementary genetics
Digitalis genetics
Escherichia coli genetics
Glucosidases genetics
Plant Proteins
Plants, Medicinal
Plants, Toxic
Subjects
Details
- Language :
- English
- ISSN :
- 0032-0781
- Volume :
- 41
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Plant & cell physiology
- Publication Type :
- Academic Journal
- Accession number :
- 11092916
- Full Text :
- https://doi.org/10.1093/pcp/pcd060