Back to Search
Start Over
A vhs-like activity of bovine herpesvirus-1.
- Source :
-
Archives of virology [Arch Virol] 2000; Vol. 145 (10), pp. 2027-46. - Publication Year :
- 2000
-
Abstract
- Bovine herpesvirus 1 (BHV-1) is a major pathogen of cattle, causing significant disease including immunosuppression in infected animals. In vitro, the surface expression of major histocompatibility complex (MHC) class I molecules, crucial for an appropriate anti-viral immune response of the host, is down-regulated by BHV-1 infection. Northern blot analyses revealed that the mRNAs for MHC class I and class II molecules were significantly down-regulated in BHV-1 infected cells, starting as early as 2 h after infection. Furthermore, mRNA expression of the two house keeping genes actin and glyceraldehyde-6-phosphate dehydrogenase (GAPDH) was also repressed after infection. This BHV-1 induced effect on cellular metabolism resembled the virion host shutoff (vhs) activity of herpes simplex virus (HSV). Similar to the HSV vhs activity, the putative BHV-1 vhs activity was not abrogated in cells infected in the presence of actinomycin D (ActD) which suggested that no viral gene expression is required for the vhs function and the putative vhs protein is associated with the virion. Sequence comparison indicated a BHV-1 open reading frame having a 60% similarity to the HSV vhs sequence. This putative BHV-1 open reading frame contained the four conserved regions of the alphaherpesvirus vhs protein. Since an HSV vhs-mutant exhibited less virulence and good immunogenicity, we suggest that a BHV-1 vhs- mutant may hold promising potential as a candidate vaccine.
- Subjects :
- Actins genetics
Actins metabolism
Animals
Base Sequence
Cattle
Cell Line
Dactinomycin pharmacology
Down-Regulation
Glyceraldehyde-3-Phosphate Dehydrogenases metabolism
Herpesvirus 1, Bovine genetics
Herpesvirus 1, Bovine pathogenicity
Histocompatibility Antigens Class I genetics
Histocompatibility Antigens Class I metabolism
Histocompatibility Antigens Class II genetics
Histocompatibility Antigens Class II metabolism
Molecular Sequence Data
RNA, Messenger metabolism
Ribonucleases
Transcription, Genetic drug effects
Viral Proteins genetics
Herpesvirus 1, Bovine physiology
Viral Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0304-8608
- Volume :
- 145
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Archives of virology
- Publication Type :
- Academic Journal
- Accession number :
- 11087090
- Full Text :
- https://doi.org/10.1007/s007050070038