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PCNA interacts with hHus1/hRad9 in response to DNA damage and replication inhibition.
- Source :
-
Oncogene [Oncogene] 2000 Nov 02; Vol. 19 (46), pp. 5291-7. - Publication Year :
- 2000
-
Abstract
- The hHus1 and several hRad proteins are involved in the control of DNA integrity checkpoints, although the mechanisms underlying these processes are unknown. Using a yeast two-hybrid system to detect protein-protein interactions, we found that human proliferating cell nuclear antigen (PCNA), a protein known to function in both DNA replication and repair, interacts with the human checkpoint-related protein Hus1 (hHus1). In human skin fibroblast cells, exposure to ionizing radiation of hydroxyurea triggers translocation of hHus1 from the cytosol to the nucleus, where it associates with PCNA as well as another checkpoint protein, hRad9. This nuclear translocation and the complex formation or hHus1 with PCNA and hRad9 correlate closely with changes in cell cycle distribution in response to radiation exposure. These results suggest that this multi-protein complex may be important for coordinating cell-cycle progression, DNA replication and repair of damaged DNA.
- Subjects :
- Active Transport, Cell Nucleus drug effects
Active Transport, Cell Nucleus radiation effects
Cell Cycle drug effects
Cell Cycle radiation effects
Cell Division drug effects
Cell Division radiation effects
Cell Line
Cell Nucleus drug effects
Cell Nucleus metabolism
Cell Nucleus radiation effects
Cells, Cultured
Cytoplasm drug effects
Cytoplasm metabolism
Cytoplasm radiation effects
Fibroblasts
Gamma Rays
Humans
Hydroxyurea pharmacology
Macromolecular Substances
Protein Binding drug effects
Protein Binding radiation effects
Schizosaccharomyces pombe Proteins
Skin
Two-Hybrid System Techniques
Cell Cycle Proteins metabolism
DNA Damage radiation effects
DNA Replication drug effects
Proliferating Cell Nuclear Antigen metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0950-9232
- Volume :
- 19
- Issue :
- 46
- Database :
- MEDLINE
- Journal :
- Oncogene
- Publication Type :
- Academic Journal
- Accession number :
- 11077446
- Full Text :
- https://doi.org/10.1038/sj.onc.1203901