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Three-dimensional characterization of interior structures of exocytotic apertures of nerve cells using atomic force microscopy.
- Source :
-
Neuroscience [Neuroscience] 2000; Vol. 101 (2), pp. 471-81. - Publication Year :
- 2000
-
Abstract
- We examined the interior structure of exocytotic apertures in synaptic vesicles of neuroblastoma x glioma hybrid cells using atomic force microscopy. The atomic force microscopy detected apertures of 50-100nm in diameter at various depths within the varicosities of these cells. We were also able to image a regular radial pattern on the wall and lump-like structures at the bottom of these apertures. In contrast, scanning electron microscopy could only detect the apertures but not the fine details of their interior. The cells examined here exhibited the same electrophysiological properties and expression of synaptophysin and syntaxin 1 as presynaptic terminals, as studied by various electrophysiological and imaging techniques. Our results indicate that atomic force microscopy allows three-dimensional viewing of the fine structures located inside exocytotic apertures in nerve cells.
- Subjects :
- Calcium metabolism
Exocytosis drug effects
Intracellular Fluid drug effects
Intracellular Fluid metabolism
Membrane Potentials drug effects
Membrane Potentials physiology
Membrane Proteins metabolism
Neurites metabolism
Neurites ultrastructure
Potassium pharmacology
Presynaptic Terminals drug effects
Presynaptic Terminals metabolism
SNARE Proteins
Synaptic Membranes drug effects
Synaptic Membranes metabolism
Synaptic Vesicles drug effects
Synaptic Vesicles metabolism
Tumor Cells, Cultured metabolism
Exocytosis physiology
Microscopy, Atomic Force methods
Presynaptic Terminals ultrastructure
Synaptic Membranes ultrastructure
Synaptic Vesicles ultrastructure
Tumor Cells, Cultured ultrastructure
Vesicular Transport Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0306-4522
- Volume :
- 101
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 11074169
- Full Text :
- https://doi.org/10.1016/s0306-4522(00)00320-1