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A new method for fast haptoglobin phenotyping and hemoglobin binding capacity calculation based on capillary zone electrophoresis.
- Source :
-
Clinical chemistry and laboratory medicine [Clin Chem Lab Med] 2000 Aug; Vol. 38 (8), pp. 715-20. - Publication Year :
- 2000
-
Abstract
- A capillary zone electrophoresis method was developed for haptoglobin (Hp) phenotyping in hemoglobin (Hb) supplemented serum. The method allows a complete resolution of the major haptoglobin phenotypes Hp 1-1, Hp 2-1, and Hp 2-2 based on the difference in charge-to-mass ratio of their Hb-Hp complexes. Identification of these phenotypes was achieved by their significant differences in migration times and their marked difference in electrophoretic pattern. Our method showed full agreement with starch gel electrophoresis. Furthermore, following neuraminidase treatment of the serum, the Hp subtypes Hp1-1FF, FS and SS could be resolved, based on the same criteria as the phenotyping. The new electrophoretic method allowed typing of the rare phenotypes Hp 2-1 modified (Hp 2-1M) and Hp Johnson. The calculated hemoglobin binding capacity of serum correlates well with the nephelometrically determined haptoglobin concentration. The new method for typing haptoglobin gives prospectives for fast haptoglobin typing and Hp 1-1 subtyping.
- Subjects :
- Electrophoresis, Capillary methods
Electrophoresis, Starch Gel
Haptoglobins chemistry
Haptoglobins genetics
Hemoglobins chemistry
Hemoglobins genetics
Humans
Isoelectric Focusing
Nephelometry and Turbidimetry methods
Neuraminidase metabolism
Phenotype
Polymorphism, Genetic
Protein Binding
Sensitivity and Specificity
Haptoglobins classification
Haptoglobins metabolism
Hemoglobins classification
Hemoglobins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1434-6621
- Volume :
- 38
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Clinical chemistry and laboratory medicine
- Publication Type :
- Academic Journal
- Accession number :
- 11071063
- Full Text :
- https://doi.org/10.1515/CCLM.2000.101