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Growth on indium-tin oxide-coated glass enhances 32P-phosphate uptake and protein labelling of adherent cells.

Authors :
Tomai E
Klein S
Firth K
Raptis L
Source :
Preparative biochemistry & biotechnology [Prep Biochem Biotechnol] 2000 Nov; Vol. 30 (4), pp. 313-20.
Publication Year :
2000

Abstract

A method to improve the efficiency of labelling of adherent cells with radioactive 32p is described. Cells are grown on a glass surface which is coated with indium-tin oxide, a commercially available, transparent material which permits excellent cell adhesion and growth. The results show that a 2 to 3-fold increase in 32p uptake by the cells can be achieved by growing cells on this material, compared to conventional tissue culture plastic.

Details

Language :
English
ISSN :
1082-6068
Volume :
30
Issue :
4
Database :
MEDLINE
Journal :
Preparative biochemistry & biotechnology
Publication Type :
Academic Journal
Accession number :
11065276
Full Text :
https://doi.org/10.1080/10826060008544970