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Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain.
- Source :
-
The Journal of cell biology [J Cell Biol] 2000 Oct 30; Vol. 151 (3), pp. 601-12. - Publication Year :
- 2000
-
Abstract
- Rab5 regulates endocytic membrane traffic by specifically recruiting cytosolic effector proteins to their site of action on early endosomal membranes. We have characterized a new Rab5 effector complex involved in endosomal fusion events. This complex includes a novel protein, Rabenosyn-5, which, like the previously characterized Rab5 effector early endosome antigen 1 (EEA1), contains an FYVE finger domain and is recruited in a phosphatidylinositol-3-kinase-dependent fashion to early endosomes. Rabenosyn-5 is complexed to the Sec1-like protein hVPS45. hVPS45 does not interact directly with Rab5, therefore Rabenosyn-5 serves as a molecular link between hVPS45 and the Rab5 GTPase. This property suggests that Rabenosyn-5 is a closer mammalian functional homologue of yeast Vac1p than EEA1. Furthermore, although both EEA1 and Rabenosyn-5 are required for early endosomal fusion, only overexpression of Rabenosyn-5 inhibits cathepsin D processing, suggesting that the two proteins play distinct roles in endosomal trafficking. We propose that Rab5-dependent formation of membrane domains enriched in phosphatidylinositol-3-phosphate has evolved as a mechanism for the recruitment of multiple effector proteins to mammalian early endosomes, and that these domains are multifunctional, depending on the differing activities of the effector proteins recruited.
- Subjects :
- Amino Acid Motifs
Amino Acid Sequence
Carrier Proteins genetics
Cathepsin D metabolism
Cell Line
Cloning, Molecular
Endosomes chemistry
Fluorescent Antibody Technique
HeLa Cells
Humans
Lysosomes chemistry
Lysosomes metabolism
Membrane Fusion
Membrane Microdomains chemistry
Membrane Microdomains metabolism
Membrane Proteins chemistry
Membrane Proteins genetics
Molecular Sequence Data
Munc18 Proteins
Nerve Tissue Proteins chemistry
Phosphatidylinositol 3-Kinases metabolism
Protein Binding
Protein Processing, Post-Translational
Protein Structure, Tertiary
Protein Transport
Qa-SNARE Proteins
Sequence Alignment
Sequence Homology, Amino Acid
Transfection
Carrier Proteins chemistry
Carrier Proteins metabolism
Endosomes metabolism
Membrane Proteins metabolism
Vesicular Transport Proteins
rab5 GTP-Binding Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9525
- Volume :
- 151
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 11062261
- Full Text :
- https://doi.org/10.1083/jcb.151.3.601