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US3 protein kinase of herpes simplex virus protects primary afferent neurons from virus-induced apoptosis in ICR mice.
- Source :
-
Neuroscience letters [Neurosci Lett] 2000 Nov 17; Vol. 294 (2), pp. 105-8. - Publication Year :
- 2000
-
Abstract
- Possible roles of the US3 gene of the herpes simplex virus (HSV) in the interaction between the virus and primary afferent neurons were examined. Neuronal apoptosis was observed in the trigeminal ganglion of mice that were infected with the wild-type (wt) of HSV-2 strain 186 and with US3-deficient mutant virus (L1BR1). In wt virus-infected mice, many HSV-immunoreactive (HSV-ir) cells were seen throughout the trigeminal ganglion, although no apoptotic change was detected. On the other hand, HSV-ir cells in L1BR1-infected mice were found only in the ophthalmic division of the trigeminal ganglions. Examination by HSV-immunohistochemistry combined with the terminal deoxynucleotidal transferase (Tdt)-mediated deoxyuridin 5'-triphosphate (dUTP) nick-end labeling (TUNEL) method showed that DNA fragmentation had occurred in almost all HSV-ir cells in the L1BRI-infected ganglion. Ultrastructurally, many viral particles were detected in apoptotic ganglionic neurons of mice infected with L1BR1. These results indicate that US3 protein kinase (US3pk) played a role in protecting HSV-infected primary afferent neurons from apoptotic cell death. The present study suggests that US3pk plays a role when HSV establishes latent infections in the sensory ganglia.
- Subjects :
- Animals
Cornea virology
Cytopathogenic Effect, Viral genetics
DNA Fragmentation
Herpesvirus 2, Human genetics
Herpesvirus 2, Human pathogenicity
Immunohistochemistry
In Situ Nick-End Labeling
Male
Mice
Mice, Inbred ICR
Neurons, Afferent pathology
Neurons, Afferent ultrastructure
Protein Serine-Threonine Kinases genetics
Specific Pathogen-Free Organisms
Trigeminal Ganglion pathology
Trigeminal Ganglion ultrastructure
Viral Proteins
Virus Latency genetics
Apoptosis
Herpesvirus 2, Human enzymology
Neurons, Afferent virology
Protein Serine-Threonine Kinases deficiency
Trigeminal Ganglion virology
Subjects
Details
- Language :
- English
- ISSN :
- 0304-3940
- Volume :
- 294
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Neuroscience letters
- Publication Type :
- Academic Journal
- Accession number :
- 11058798
- Full Text :
- https://doi.org/10.1016/s0304-3940(00)01554-8