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Efavirenz is a potent nonnucleoside reverse transcriptase inhibitor of HIV type 1 replication in microglia in vitro.
- Source :
-
AIDS research and human retroviruses [AIDS Res Hum Retroviruses] 2000 Oct 10; Vol. 16 (15), pp. 1527-37. - Publication Year :
- 2000
-
Abstract
- The objective of this study was to determine whether reverse transcriptase inhibitors (RTIs) could decrease viral replication in microglia. Human microglia obtained from individuals undergoing temporal lobectomy were cultured and infected with HIV-1 isolates from the central nervous system (CNS) as previously described (Strizki JM, et al. J Virol 1996;70:7654-7662). These microglial cultures were treated with one of three nucleoside RTIs (NRTIs) or with efavirenz, a nonnucleoside RTI (NNRTI), at various time points before and during HIV-1 infection. The drug levels sufficient to provide > 90% inhibition of microglial HIV replication (IC90) were determined by comparison of p24(gag) release in the cultures among treated and untreated microglia. Infectious virus released from the infected cultures was also measured with U373-MAGI-CCR5 cells. Efavirenz, an NNRTI, blocked HIV-1(DS-br) infection of microglia with an IC(90) of 0.7-7 nM. This value is similar to the efavirenz IC(90) values for inhibition of laboratory and clinical isolates in lymphocytes, is 2-3 logs lower than the IC90 values of AZT and d4T, and is 1-2 logs lower than that of ddC in microglia. Efavirenz also inhibited infection with other neurotropic isolates, and with viruses isolated from other compartments that also replicated well in microglia. Thus, efavirenz is a potent inhibitor of HIV-1 infection in microglia. Furthermore, efavirenz IC(90) drug levels are present in the cerebrospinal fluid (CSF) of patients taking this once daily NNRTI.
- Subjects :
- Adult
Alkynes
Benzoxazines
Cells, Cultured
Coculture Techniques
Cyclopropanes
HIV-1 growth & development
HIV-1 physiology
Humans
Microglia cytology
Neuroglia cytology
Nucleosides
Stavudine pharmacology
Zalcitabine pharmacology
Zidovudine pharmacology
Anti-HIV Agents pharmacology
HIV-1 drug effects
Microglia virology
Oxazines pharmacology
Reverse Transcriptase Inhibitors pharmacology
Virus Replication drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0889-2229
- Volume :
- 16
- Issue :
- 15
- Database :
- MEDLINE
- Journal :
- AIDS research and human retroviruses
- Publication Type :
- Academic Journal
- Accession number :
- 11054266
- Full Text :
- https://doi.org/10.1089/088922200750006056