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Determination of biotin and folate in infant formula and milk by optical biosensor-based immunoassay.
- Source :
-
Journal of AOAC International [J AOAC Int] 2000 Sep-Oct; Vol. 83 (5), pp. 1141-8. - Publication Year :
- 2000
-
Abstract
- Biomolecular interaction analysis was evaluated for the automated analysis of biotin- and folate-supplemented infant formulas and milk powders. The technique was configured as a biosensor-based, nonlabeled inhibition immunoassay using monoclonal antibodies raised against analyte-conjugate. Sample extraction conditions were optimized and antibodies were evaluated for cross-reactivity. Performance parameters included a quantitation range of 2-70 ng/mL, recoveries of 86-102%, agreement against assigned reference values for National Institute of Standards and Technology Standard Reference Material 1846, between-laboratory reproducibility relative standard deviation of 9.1% for biotin and 8.1% for folate, respectively, and equivalence against reference microbiological assay methods for both analytes.
Details
- Language :
- English
- ISSN :
- 1060-3271
- Volume :
- 83
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of AOAC International
- Publication Type :
- Academic Journal
- Accession number :
- 11048855