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Differential expression of two glucuronyltransferases synthesizing HNK-1 carbohydrate epitope in the sublineages of the rat myogenic progenitors.
- Source :
-
Mechanisms of development [Mech Dev] 2000 Nov; Vol. 98 (1-2), pp. 145-9. - Publication Year :
- 2000
-
Abstract
- HNK-1 epitope is a cell-surface carbohydrate mediating various cell-cell or cell-substrate interactions. We found HNK-1 epitope in longitudinally arrayed fibers in the subpopulation of the epaxial myotome, and hypaxial myoblasts migrating into the limb bud in the rat embryo. We next investigated the expression patterns of genes encoding two glucuronyltransferases (GlcAT-P, GlcAT-D) and sulfotransferase (Sul-T), which are required for biosynthesis of HNK-1 epitope. GlcAT-P gene was expressed in the non-migrating longitudinal fibers, whereas GlcAT-D gene was expressed in the migrating myoblasts in the limb bud. Sul-T gene expression was ubiquitously observed in all these myogenic populations. Thus, differential expression of GlcAT genes may relate to the epaxial/hypaxial or migrating/non-migrating myoblast lineages.
- Subjects :
- Animals
Epitopes biosynthesis
Gene Expression Regulation, Developmental
Glucuronosyltransferase metabolism
In Situ Hybridization
Muscles embryology
Muscles enzymology
Muscles immunology
Rats
Rats, Sprague-Dawley
Stem Cells enzymology
Stem Cells immunology
Sulfotransferases genetics
CD57 Antigens biosynthesis
Glucuronosyltransferase genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0925-4773
- Volume :
- 98
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Mechanisms of development
- Publication Type :
- Academic Journal
- Accession number :
- 11044619
- Full Text :
- https://doi.org/10.1016/s0925-4773(00)00449-4