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The matrix metalloproteinase-1 (MMP-1) expression in the human endometrium is inversely regulated by interleukin-1 alpha and sex steroids.
- Source :
-
Ceska gynekologie [Ceska Gynekol] 2000 Jul; Vol. 65 (4), pp. 211-5. - Publication Year :
- 2000
-
Abstract
- Objective: To investigate the regulation of perimenstrual MMP-1 expression in human endometrium.<br />Design: In vitro study utilizing epithelial-stromal co-cultures.<br />Setting: Cell Biology Unit, International Institute of Cellular and Molecular Pathology, and Departments of Pathology and Gynecology, Saint-Luc University Clinics, Louvain University Medical School, Brussels, Belgium.<br />Methods: Contact-dependent and contact-independent co-cultures were established and resulting MMP-1 gene and protein expression was analyzed by RNase protection assays and soluble-collagen assays.<br />Results: MMP-1 expression in endometrial fibroblasts is markedly stimulated by epithelial cell-conditioned medium. This stimulation can be prevented by antibodies directed against interleukin 1 alpha (IL-1 alpha). Ovarian steroids inhibit MMP-1 production by IL-1 alpha-stimulated fibroblasts in vitro.<br />Conclusion: Taken together, our results suggest that epithelium-derived IL-1 alpha is the most important paracrine induced of MMP-1 in endometrial fibroblasts. However, IL-1 alpha-stimulated MMP-1 production in the human endometrium is effectively blocked by ovarian steroids. We believe that this mechanism responsible for the MMP-1 repression that occurs when systemic sex steroid concentrations are high and the MMP-1 production and activity during the perimenstrual phase when estrogen and progesterone concentrations are low.
- Subjects :
- Coculture Techniques
Culture Media, Conditioned
Endometrium cytology
Epithelium metabolism
Female
Fibroblasts metabolism
Gonadal Steroid Hormones pharmacology
Humans
Interleukin-1 pharmacology
Menstruation
Endometrium metabolism
Gonadal Steroid Hormones physiology
Interleukin-1 physiology
Matrix Metalloproteinase 1 biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1210-7832
- Volume :
- 65
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Ceska gynekologie
- Publication Type :
- Academic Journal
- Accession number :
- 11039223