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Dnm1p GTPase-mediated mitochondrial fission is a multi-step process requiring the novel integral membrane component Fis1p.
- Source :
-
The Journal of cell biology [J Cell Biol] 2000 Oct 16; Vol. 151 (2), pp. 367-80. - Publication Year :
- 2000
-
Abstract
- Yeast Dnm1p is a soluble, dynamin-related GTPase that assembles on the outer mitochondrial membrane at sites where organelle division occurs. Although these Dnm1p-containing complexes are thought to trigger constriction and fission, little is known about their composition and assembly, and molecules required for their membrane recruitment have not been isolated. Using a genetic approach, we identified two new genes in the fission pathway, FIS1 and FIS2. FIS1 encodes a novel, outer mitochondrial membrane protein with its amino terminus exposed to the cytoplasm. Fis1p is the first integral membrane protein shown to participate in a eukaryotic membrane fission event. In a related study (Tieu, Q., and J. Nunnari. 2000. J. Cell Biol. 151:353-365), it was shown that the FIS2 gene product (called Mdv1p) colocalizes with Dnm1p on mitochondria. Genetic and morphological evidence indicate that Fis1p, but not Mdv1p, function is required for the proper assembly and distribution of Dnm1p-containing fission complexes on mitochondrial tubules. We propose that mitochondrial fission in yeast is a multi-step process, and that membrane-bound Fis1p is required for the proper assembly, membrane distribution, and function of Dnm1p-containing complexes during fission.
- Subjects :
- Dynamins
GTP Phosphohydrolases genetics
Genes, Fungal
Intracellular Membranes
Membrane Fusion
Membrane Proteins genetics
Mitochondrial Proteins
Models, Biological
Multigene Family
Mutation
Protein Conformation
Saccharomyces cerevisiae physiology
Saccharomyces cerevisiae Proteins
Suppression, Genetic
GTP Phosphohydrolases metabolism
Membrane Proteins metabolism
Mitochondria physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9525
- Volume :
- 151
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 11038183
- Full Text :
- https://doi.org/10.1083/jcb.151.2.367