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Localization of transcription factor AP-1 family proteins in ameloblast nuclei of the rat incisor.
- Source :
-
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society [J Histochem Cytochem] 2000 Nov; Vol. 48 (11), pp. 1511-20. - Publication Year :
- 2000
-
Abstract
- We examined by immunocytochemistry the localization of the AP-1 family proteins c-Jun, JunB, JunD, c-Fos, FosB, Fra-1, and Fra-2 in rat incisor ameloblasts. Most of the antibodies against AP-1 family proteins, except for c-Fos-specific antibody, labeled ameloblast nuclei. The labeling intensity of the c-Jun, JunD, and Fra-2 antibodies was stronger than that of JunB, FosB, and Fra-1. Antibody reactivities of c-Jun, JunD, and Fra-2 were greatly enhanced during or after the transition zone. Furthermore, c-Jun antibodies labeled maturation ameloblasts in a cyclic pattern, which was correlated with ameloblast modulation. Disruption of ameloblast modulation by colchicine injection resulted in greatly decreased reactivity of the c-Jun antibody in the ameloblast nuclei of the maturation zone. Phospho-specific antibodies to c-Jun labeled ameloblast nuclei only weakly throughout the secretion, transition, and maturation zones. These results suggest that the stage-specific localization of AP-1 in ameloblasts is closely related to tooth enamel formation.
- Subjects :
- Ameloblasts ultrastructure
Animals
Antibodies
Cell Nucleus metabolism
Colchicine pharmacology
Immunohistochemistry
Male
Proto-Oncogene Proteins c-fos immunology
Proto-Oncogene Proteins c-fos metabolism
Proto-Oncogene Proteins c-jun immunology
Proto-Oncogene Proteins c-jun metabolism
Rats
Rats, Wistar
Transcription Factor AP-1 immunology
Ameloblasts metabolism
Incisor metabolism
Transcription Factor AP-1 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1554
- Volume :
- 48
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
- Publication Type :
- Academic Journal
- Accession number :
- 11036094
- Full Text :
- https://doi.org/10.1177/002215540004801108