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Identification of five different Patr class I molecules that bind HLA supertype peptides and definition of their peptide binding motifs.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2000 Oct 15; Vol. 165 (8), pp. 4414-22. - Publication Year :
- 2000
-
Abstract
- We have sequenced the Pan troglodytes class I (Patr) molecules from three common chimpanzees and expressed them as single molecules in a class I-deficient cell line. These lines were utilized to obtain purified class I molecules to define the peptide binding motifs associated with five different Patr molecules. Based on these experiments, as well as analysis of the predicted structure of the B and F polymorphic MHC pockets, we classified five Patr molecules (Patr-A*0101, Patr-B*0901, Patr-B*0701, Patr-A*0602, and Patr-B*1301) within previously defined supertype specificities associated with HLA class I molecules (HLA-A3, -B7, -A1, and -A24 supertypes). The overlap in the binding repertoire between specific HLA and Patr class I molecules was in the range of 33 to 92%, depending on the particular Patr molecule as assessed by the binding of HIV-, hepatitis B virus-, and hepatitis C virus-derived epitopes. Finally, live cell binding assays of nine chimpanzee-derived B cell lines demonstrated that HLA supertype peptides bound to Patr class I molecules with frequencies in the 20-50% range.
- Subjects :
- Alleles
Amino Acid Motifs genetics
Amino Acid Motifs immunology
Animals
B-Lymphocytes metabolism
Cell Line, Transformed
Genes, MHC Class I
HLA-A1 Antigen genetics
HLA-A1 Antigen metabolism
HLA-A3 Antigen genetics
HLA-A3 Antigen metabolism
HLA-B Antigens genetics
HLA-B Antigens metabolism
HLA-B7 Antigen genetics
HLA-B7 Antigen metabolism
Histocompatibility Antigens Class I genetics
Histocompatibility Antigens Class I isolation & purification
Humans
Oligopeptides chemical synthesis
Pan troglodytes genetics
Protein Binding genetics
Protein Binding immunology
Sequence Analysis, DNA
Transfection
HLA Antigens metabolism
Histocompatibility Antigens Class I metabolism
Oligopeptides immunology
Oligopeptides metabolism
Pan troglodytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 165
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 11035079
- Full Text :
- https://doi.org/10.4049/jimmunol.165.8.4414