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Requirements for bone marrow-derived antigen-presenting cells in priming cytotoxic T cell responses to intracellular pathogens.
- Source :
-
The Journal of experimental medicine [J Exp Med] 2000 Oct 16; Vol. 192 (8), pp. 1135-42. - Publication Year :
- 2000
-
Abstract
- Bone marrow (BM)-derived antigen-presenting cells (APCs) are potent stimulators of T cell immune responses. We investigated the requirements for antigen presentation by these cells in priming cytotoxic T lymphocyte (CTL) responses to intracellular bacterial and viral pathogens. [Parent-->F(1)] radiation BM chimeras were constructed using C57BL/6 donors and (C57BL/6 x BALB/c)F(1) recipients. Infection of chimeric mice with either Listeria monocytogenes or vaccinia virus expressing the nucleoprotein (NP) antigen from lymphocytic choriomeningitis virus (LCMV) primed H2-D(b)-restricted, but not H2-K(d)-restricted CTL responses, demonstrating the requirement for BM-derived APCs for successful priming of CTL responses to these pathogens. Surprisingly, this did not hold true for chimeric mice infected with LCMV itself. LCMV-infected animals developed strong CTL responses specific for both H2-D(b)- and H2-L(d)-restricted NP epitopes. These findings indicate that in vivo priming of CTL responses to LCMV is remarkably insensitive to deficiencies in antigen presentation by professional BM-derived APCs.
- Subjects :
- Animals
Antigen-Presenting Cells cytology
Bone Marrow Cells cytology
Chimera
Female
Flow Cytometry
Listeria monocytogenes immunology
Lymphocytic choriomeningitis virus immunology
Major Histocompatibility Complex
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Nucleoproteins immunology
Vaccinia virus immunology
Antigen-Presenting Cells immunology
Bone Marrow Cells immunology
Cytotoxicity, Immunologic
Listeriosis immunology
Lymphocytic Choriomeningitis immunology
T-Lymphocytes, Cytotoxic immunology
Vaccinia immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1007
- Volume :
- 192
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- The Journal of experimental medicine
- Publication Type :
- Academic Journal
- Accession number :
- 11034603
- Full Text :
- https://doi.org/10.1084/jem.192.8.1135