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Effect of IGFBP-derived peptides on incorporation of(35)SO(4)into proteoglycans.
- Source :
-
Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society [Growth Horm IGF Res] 2000 Aug; Vol. 10 (4), pp. 224-9. - Publication Year :
- 2000
-
Abstract
- 18 amino acid peptides from the C-terminal region of IGFBP-3, -5 (P3, P5), increased the incorporation of(35)SO(4)into proteoglycans in endothelial cells with greater stimulation in large vessel than microvessel cells. The homologous region of IGFBP-6 (P6) also stimulated sulfate uptake, but less potently than P3 and P5. P6 variants were synthesized with one or two amino acids changed to the basic amino acid in the equivalent position of P3. The P6 variants with one additional basic amino acid behaved similarly to P6. The P6 mutant with two altered amino acids was equipotent to P3. P3F, a scrambled version of P3 was less effective than P3. P3, P5, P6, P3F and all P6 variants all stimulated glucose uptake, which occurred only in microvessel cells. P1, P2, P4, and equimolar intact IGFBP-3 stimulated neither glucose uptake nor sulfate incorporation. Thus, C-terminal basic portions of IGFBP-3, -5 and -6 alter two specific functions of endothelial cells with sufficient differences to suggest mediation by distinct mechanisms.<br /> (Copyright 2000 Harcourt Publishers Ltd.)
- Subjects :
- Amino Acid Sequence
Animals
Cattle
Cells, Cultured
Endothelium, Vascular cytology
Endothelium, Vascular drug effects
Endothelium, Vascular metabolism
Insulin-Like Growth Factor Binding Protein 3 chemistry
Insulin-Like Growth Factor Binding Protein 3 genetics
Insulin-Like Growth Factor Binding Protein 3 pharmacology
Insulin-Like Growth Factor Binding Protein 5 chemistry
Insulin-Like Growth Factor Binding Protein 5 genetics
Insulin-Like Growth Factor Binding Protein 5 pharmacology
Insulin-Like Growth Factor Binding Protein 6 chemistry
Insulin-Like Growth Factor Binding Protein 6 genetics
Insulin-Like Growth Factor Binding Protein 6 pharmacology
Insulin-Like Growth Factor Binding Proteins chemistry
Insulin-Like Growth Factor Binding Proteins genetics
Molecular Sequence Data
Peptide Fragments chemistry
Peptide Fragments genetics
Peptide Fragments pharmacology
Insulin-Like Growth Factor Binding Proteins pharmacology
Proteoglycans metabolism
Sulfates metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1096-6374
- Volume :
- 10
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society
- Publication Type :
- Academic Journal
- Accession number :
- 11032706
- Full Text :
- https://doi.org/10.1054/ghir.2000.0158