A fruiting body tissue method for efficient Agrobacterium-mediated transformation of Agaricus bisporus.

Authors :: Chen X
Stone M
Schlagnhaufer C
Romaine CP
Source :: Applied and environmental microbiology [Appl Environ Microbiol] 2000 Oct; Vol. 66 (10), pp. 4510-3.
Publication Year :: 2000
Abstract: We describe a modified Agrobacterium-mediated method for the efficient transformation of Agaricus bisporus. Salient features of this procedure include cocultivation of Agrobacterium and fruiting body gill tissue and use of a vector with a homologous promoter. This method offers new prospects for the genetic manipulation of this commercially important mushroom species.

Subjects :: Agaricus growth & development
DNA Primers
Drug Resistance, Microbial genetics
Genetic Vectors
Hygromycin B pharmacology
Plasmids
Polymerase Chain Reaction
Promoter Regions, Genetic
Agaricus genetics
Agrobacterium tumefaciens genetics
Cinnamates
Hygromycin B analogs & derivatives
Transformation, Genetic

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