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Energy metabolism in graded perinatal asphyxia of the rat.
- Source :
-
Life sciences [Life Sci] 2000 Jun 16; Vol. 67 (4), pp. 421-35. - Publication Year :
- 2000
-
Abstract
- Although information on energy metabolism during hypoxemic-ischemic states is abundant, data on perinatal asphyxia (PA) are limited. As results from hypoxia-ischemia cannot be directly extrapolated to PA, a clinical entity characterized by acidosis, hypoxemia and hypercapnia, we decided to use a rat model of graded PA during delivery. Cesarean section was performed at the 21st day of gestation and the pups, still in the uterus horns, were asphyxiated from 0 to 20 minutes. In this model survival decreases with the length of asphyxia. Early changes of energy-rich phosphates in brain, heart and kidney were determined by HPLC. ATP and phosphocreatine gradually decreased with the length of asphyxia, with highest ATP depletion rate occurring in the kidney. ATP: brain 1.39 +/- 0.71 (0 min) to 0.06 microM/g wwt (20 min); heart 4.73 +/- 0.34 (0 min) to 1.08 +/- 0.47 (20 min); kidney 1.62 +/- 0.11 (0 min) to 0.02 +/- 0.02 (20 min). Phosphocreatine: brain 1.65 +/- 0.68 (0 min) to 0.51 +/- 0.45 microM/g (20 min); heart 6.98 +/- 0.38 (0 min) to 6.17 +/- 1.07 (20 min); kidney 8.23 +/- 0.86 (0 min) to 3.76 +/- 0.54 (20 min). We present data on energy derangement in a rat model of PA, closely resembling the clinical situation, showing that energy depletion precedes cell damage and death.
- Subjects :
- Animals
Animals, Newborn
Blood Gas Analysis
Brain metabolism
Chromatography, High Pressure Liquid
Disease Models, Animal
Female
Hydrogen-Ion Concentration
Kidney metabolism
Lactic Acid metabolism
Myocardium metabolism
Phosphocreatine metabolism
Pregnancy
Rats
Rats, Sprague-Dawley
Adenine Nucleotides metabolism
Asphyxia metabolism
Energy Metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0024-3205
- Volume :
- 67
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 11003052
- Full Text :
- https://doi.org/10.1016/s0024-3205(00)00630-5