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Differentiation of rat pheochromocytoma cells by fomitellic acids, specific DNA polymerase inhibitors.

Authors :
Obara Y
Nakahata N
Mizushina Y
Sugawara F
Sakaguchi K
Ohizumi Y
Source :
Life sciences [Life Sci] 2000 Aug 18; Vol. 67 (13), pp. 1659-65.
Publication Year :
2000

Abstract

Fomitellic acid (FA) A and B are specific inhibitors of DNA polymerase alpha and beta. They showed cytotoxicity against rat pheochromocytoma cells (PC-12 cells) in a concentration-dependent manner. However, after PC-12 cells were cultivated with low concentrations of FAs, the cells extended neurites in greater degree similar to the cells cultivated with nerve growth factor. Another DNA polymerase alpha inhibitor, aphidicolin, also induced neurite outgrowth. Furthermore, PC-12 cells were strongly immunostained with anti-alpha-tubulin or anti-tau antibody after the treatment with FAs. These results suggest that weak inhibition of DNA polymerase activity induces the neurite outgrowth in PC-12 cells.

Details

Language :
English
ISSN :
0024-3205
Volume :
67
Issue :
13
Database :
MEDLINE
Journal :
Life sciences
Publication Type :
Academic Journal
Accession number :
10983859
Full Text :
https://doi.org/10.1016/s0024-3205(00)00742-6