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Differentiation of rat pheochromocytoma cells by fomitellic acids, specific DNA polymerase inhibitors.
- Source :
-
Life sciences [Life Sci] 2000 Aug 18; Vol. 67 (13), pp. 1659-65. - Publication Year :
- 2000
-
Abstract
- Fomitellic acid (FA) A and B are specific inhibitors of DNA polymerase alpha and beta. They showed cytotoxicity against rat pheochromocytoma cells (PC-12 cells) in a concentration-dependent manner. However, after PC-12 cells were cultivated with low concentrations of FAs, the cells extended neurites in greater degree similar to the cells cultivated with nerve growth factor. Another DNA polymerase alpha inhibitor, aphidicolin, also induced neurite outgrowth. Furthermore, PC-12 cells were strongly immunostained with anti-alpha-tubulin or anti-tau antibody after the treatment with FAs. These results suggest that weak inhibition of DNA polymerase activity induces the neurite outgrowth in PC-12 cells.
- Subjects :
- Animals
DNA Polymerase I antagonists & inhibitors
DNA Polymerase beta antagonists & inhibitors
Enzyme Inhibitors toxicity
Growth Cones drug effects
Inhibitory Concentration 50
Neurites drug effects
PC12 Cells
Rats
Terpenes toxicity
Cell Differentiation drug effects
Enzyme Inhibitors pharmacology
Terpenes pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0024-3205
- Volume :
- 67
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 10983859
- Full Text :
- https://doi.org/10.1016/s0024-3205(00)00742-6