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Investigation of the susceptibility of equine autonomic neuronal cell lines, clonally derived from the same paravertebral ganglion, to toxic plasma from equine dysautonomia (grass sickness) cases.
- Source :
-
Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2000 Oct; Vol. 14 (5), pp. 459-65. - Publication Year :
- 2000
-
Abstract
- In the autonomic nervous system (ANS) of equine grass sickness (GS) cases, some neurones show abnormal changes while neighbouring neurones are unaffected. To test whether noradrenergic neurones showed variable susceptibility to the GS toxin in culture, clonally-derived populations isolated from the same fetal thoracic sympathetic chain ganglion were challenged with plasma from GS cases previously shown to induce ANS damage when injected into normal horses. During the early stages of exposure to toxic plasma, cells within a clonal population showed variable susceptibility ranging from no obvious effect to characteristic patterns of pathology. However, after 3 days of exposure to toxic plasma all cells were killed. Dose response analysis on selected clonal populations showed no significant difference in TD(50) values.
- Subjects :
- Animals
Antigens, Polyomavirus Transforming metabolism
Autonomic Nervous System metabolism
Autonomic Nervous System Diseases blood
Cell Line
Clone Cells
Dopamine beta-Hydroxylase metabolism
Fluorescent Antibody Technique, Indirect
Ganglia, Sympathetic metabolism
Glial Fibrillary Acidic Protein metabolism
Horses
Neurons metabolism
Neuropeptide Y metabolism
Thorax innervation
Autonomic Nervous System pathology
Autonomic Nervous System Diseases veterinary
Ganglia, Sympathetic pathology
Horse Diseases blood
Neurons pathology
Plasma physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0887-2333
- Volume :
- 14
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Toxicology in vitro : an international journal published in association with BIBRA
- Publication Type :
- Academic Journal
- Accession number :
- 10963962
- Full Text :
- https://doi.org/10.1016/s0887-2333(00)00037-0