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Genetic analysis of enterovirus 71 isolated from fatal and non-fatal cases of hand, foot and mouth disease during an epidemic in Taiwan, 1998.
- Source :
-
Virus research [Virus Res] 2000 Jul; Vol. 68 (2), pp. 127-36. - Publication Year :
- 2000
-
Abstract
- A large scale outbreak of hand-foot-and-mouth disease (HFMD) occurred in Taiwan in 1998, in which more than 80 children died of shock syndrome with pulmonary edema/hemorrhage. Enterovirus 71 was implicated as the cause of this outbreak. In order to understand the virological basis responsible for mortality on this scale, nucleotide sequences of VP1 that is important for serotypic specificity, and the 5'-non-coding region (5'-NCR) that is important for replication efficiency, were analyzed comparatively. Phylogenetic analysis of both VP1 and 5'-NCR of nine EV71 isolates derived from specimens of fatal patients and seven isolates derived from uncomplicated HFMD patients showed that all but one isolate fell into genotype B. The one distinct isolate from a case of uncomplicated HFMD belonged to genotype C that was clustered along with one isolate from Taiwan in 1986. Complete sequence analysis of two selected isolates, one from the spinal cord of a fatal case and one from the vesicle fluid of a patient with mild HFMD, confirmed a high degree (97-100%) of identity in nucleotide sequence throughout the entire genome, except focal regions of 3C and 3'-NCR where the nucleotide homology was 90-91%. The identity of the deduced amino acid sequence in the 3C region that encodes viral proteinase dropped further to 86%, a result of missense mutations at the first nucleotide position of many codons.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Capsid Proteins
Cell Line
Child
DNA, Viral
Enterovirus classification
Enterovirus isolation & purification
Hand, Foot and Mouth Disease epidemiology
Haplorhini
Humans
Molecular Sequence Data
Sequence Analysis
Sequence Homology, Amino Acid
Taiwan epidemiology
5' Untranslated Regions
Capsid genetics
Disease Outbreaks
Enterovirus genetics
Hand, Foot and Mouth Disease virology
Subjects
Details
- Language :
- English
- ISSN :
- 0168-1702
- Volume :
- 68
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Virus research
- Publication Type :
- Academic Journal
- Accession number :
- 10958984
- Full Text :
- https://doi.org/10.1016/s0168-1702(00)00162-3