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Prenylation of the floral transcription factor APETALA1 modulates its function.
- Source :
-
The Plant cell [Plant Cell] 2000 Aug; Vol. 12 (8), pp. 1257-66. - Publication Year :
- 2000
-
Abstract
- The Arabidopsis MADS box transcription factor APETALA1 (AP1) was identified as a substrate for farnesyltransferase and shown to be farnesylated efficiently both in vitro and in vivo. AP1 regulates the transition from inflorescence shoot to floral meristems and the development of sepals and petals. AP1 fused to green fluorescent protein (GFP) retained transcription factor activity and directed the expected terminal flower phenotype when ectopically expressed in transgenic Arabidopsis. However, ap1mS, a farnesyl cysteine-acceptor mutant of AP1, as well as the GFP-ap1mS fusion protein failed to direct the development of compound terminal flowers but instead induced novel phenotypes when ectopically expressed in Arabidopsis. Similarly, compound terminal flowers did not develop in era1-2 transformants that ectopically expressed AP1. Together, the results demonstrate that AP1 is a target of farnesyltransferase and suggest that farnesylation alters the function and perhaps specificity of the transcription factor.
- Subjects :
- Alkyl and Aryl Transferases genetics
Alkyl and Aryl Transferases metabolism
Amino Acid Motifs
Amino Acid Sequence
Arabidopsis enzymology
Arabidopsis genetics
Arabidopsis Proteins
Farnesyltranstransferase
Genes, Plant genetics
Homeodomain Proteins genetics
MADS Domain Proteins
Molecular Sequence Data
Mutation genetics
Phenotype
Plant Growth Regulators chemistry
Plant Growth Regulators metabolism
Plant Proteins genetics
Plant Structures genetics
Plant Structures growth & development
Plants, Genetically Modified
Recombinant Fusion Proteins chemistry
Recombinant Fusion Proteins metabolism
Sequence Alignment
Transcription Factors genetics
Arabidopsis growth & development
Homeodomain Proteins chemistry
Homeodomain Proteins metabolism
Plant Proteins chemistry
Plant Proteins metabolism
Protein Prenylation
Transcription Factors chemistry
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1040-4651
- Volume :
- 12
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- The Plant cell
- Publication Type :
- Academic Journal
- Accession number :
- 10948247
- Full Text :
- https://doi.org/10.1105/tpc.12.8.1257