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Expression and secretion of scytalidopepsin B, an acid protease from Scytalidium lignicolum, in yeast.
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2000 Jul; Vol. 64 (7), pp. 1542-6. - Publication Year :
- 2000
-
Abstract
- An expression and secretion system for scytalidopepsin B, an acid protease from Scytalidium lignicolum, was constructed in yeast. Saccharomyces cerevisiae AH22 was transformed with an yeast-E. coli shuttle vector, pAM82, in which an yeast invertase signal segment and the cDNA encoding the pro- and mature enzyme regions were inserted. The transformant was found to secret a pepstatin-insensitive acid protease, when cultured aerobically in a low phosphate (Pi) medium. Amino terminal amino acid sequencing analysis indicated that the recombinant acid protease was accurately processed and secreted as a mature form.
Details
- Language :
- English
- ISSN :
- 0916-8451
- Volume :
- 64
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 10945280
- Full Text :
- https://doi.org/10.1271/bbb.64.1542