Back to Search
Start Over
Decreased expression of p57(KIP2)mRNA in human bladder cancer.
- Source :
-
British journal of cancer [Br J Cancer] 2000 Sep; Vol. 83 (5), pp. 626-31. - Publication Year :
- 2000
-
Abstract
- To identify targets of genetic and epigenetic alterations on chromosome 11p15.5 in human bladder cancer, expression of the imprinted KIP2, IGF2 and H19 genes was studied by quantitative RT-PCR in 24 paired samples of urothelial carcinomas and morphologically normal mucosa obtained by cystectomy, and in bladder carcinoma cell lines. The most frequent alteration in tumour tissue was decreased expression of KIP2 identified in 9/24 (37%) specimens. Decreased IGF2 and H19 mRNA levels were found in five (21%) and three (13%) tumours, respectively. One tumour each overexpressed IGF2 and H19. Loss of H19 expression was only found associated with loss of KIP2 expression, whereas decreased expression of IGF2 mRNA occurred independently. Almost all bladder carcinoma cell lines showed significant changes in the expression of at least one gene with diminished expression of KIP2 mRNA as the most frequent alteration. IGF2 mRNA levels were diminished in several lines, but increased in others. The KIP2 gene could be an important target of genetic and epigenetic alterations in bladder cancer affecting the maternal chromosome 11p15.5. However, reminiscent of the situation in Wilms' tumours, expression of the IGF2 gene on the paternal chromosome can also be disturbed in bladder cancers.<br /> (Copyright 2000 Cancer Research Campaign.)
- Subjects :
- Aged
Carcinoma genetics
Carcinoma metabolism
Chromosomes, Human, Pair 11
Cyclin-Dependent Kinase Inhibitor p57
Female
Humans
Insulin-Like Growth Factor II biosynthesis
Insulin-Like Growth Factor II genetics
Loss of Heterozygosity
Male
Middle Aged
Mucous Membrane metabolism
Muscle Proteins biosynthesis
Muscle Proteins genetics
Nuclear Proteins genetics
RNA, Long Noncoding
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
Urinary Bladder metabolism
Urinary Bladder Neoplasms genetics
Urothelium metabolism
Nuclear Proteins biosynthesis
RNA, Messenger biosynthesis
RNA, Untranslated
Urinary Bladder Neoplasms metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0007-0920
- Volume :
- 83
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- British journal of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 10944603
- Full Text :
- https://doi.org/10.1054/bjoc.2000.1298