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Discriminating between homodimeric and monomeric proteins in the crystalline state.
- Source :
-
Proteins [Proteins] 2000 Oct 01; Vol. 41 (1), pp. 47-57. - Publication Year :
- 2000
-
Abstract
- Scores calculated from intermolecular contacts of proteins in the crystalline state are used to differentiate monomeric and homodimeric proteins, by classification into two categories separated by a cut-off score value. The generalized classification error is estimated by using bootstrap re-sampling on a nonredundant set of 172 water-soluble proteins whose prevalent quaternary state in solution is known to be either monomeric or homodimeric. A statistical potential, based on atom-pair frequencies across interfaces observed with homodimers, is found to yield an error rate of 12.5%. This indicates a small but significant improvement over the measure of solvent accessible surface area buried in the contact interface, which achieves an error rate of 15.4%. A further modification of the latter parameter relating the two most extensive contacts of the crystal results in an even lower error rate of 11.1%.
- Subjects :
- Crystallization
Dimerization
Thermodynamics
Proteins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0887-3585
- Volume :
- 41
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Proteins
- Publication Type :
- Academic Journal
- Accession number :
- 10944393
- Full Text :
- https://doi.org/10.1002/1097-0134(20001001)41:1<47::aid-prot80>3.3.co;2-#