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Expression of cyclo-oxygenase-2 in human atherosclerotic carotid arteries.
- Source :
-
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery [Eur J Vasc Endovasc Surg] 2000 Aug; Vol. 20 (2), pp. 146-52. - Publication Year :
- 2000
-
Abstract
- Objectives: the preventive effect of acetylsalicylic acid in cardiovascular disease may be due to inhibition of platelet aggregation mediated by COX-1, but may in addition be due to anti-inflammatory effects by inhibition of COX-2. The objective of this study was to analyse the expression of COX-1 and COX-2 in atherosclerotic and healthy vascular walls.<br />Design: the expression COX-1 and COX-2 was analysed in biopsies from human atherosclerotic carotid arteries and from healthy mammary arteries and saphenous veins.<br />Materials: vascular biopsies were obtained from patients undergoing carotid endarterectomy or coronary bypass surgery.<br />Methods: RT-PCR was used for mRNA analysis and for localization of proteins we used immunohistochemistry.<br />Results: COX-2 was found in atherosclerotic plaques: in macrophages, in some smooth muscle cells and in endothelial cells of small vessels in the lesions. In non-atherosclerotic blood vessels, COX-2 was detected in the endothelium of the vasa vasorum in the adventitia. COX-1 was found in the endothelium in healthy and in atherosclerotic vessels.<br />Conclusions: the expression of COX-2 by inflammatory and vascular cells in atherosclerotic arteries suggests that products of this enzyme may be important in the pathogenesis of atherosclerosis.<br /> (Copyright 2000 Harcourt Publishers Ltd.)
- Subjects :
- Arteriosclerosis drug therapy
Arteriosclerosis immunology
Aspirin pharmacology
Biopsy
Carotid Artery Diseases drug therapy
Carotid Artery Diseases immunology
Case-Control Studies
Cyclooxygenase 1
Cyclooxygenase 2
Cyclooxygenase 2 Inhibitors
Cyclooxygenase Inhibitors pharmacology
Endothelium, Vascular enzymology
Humans
Immunohistochemistry
Inflammation
Isoenzymes drug effects
Isoenzymes genetics
Isoenzymes immunology
Macrophages enzymology
Membrane Proteins
Muscle, Smooth, Vascular enzymology
Platelet Aggregation Inhibitors pharmacology
Prostaglandin-Endoperoxide Synthases drug effects
Prostaglandin-Endoperoxide Synthases genetics
Prostaglandin-Endoperoxide Synthases immunology
Reverse Transcriptase Polymerase Chain Reaction
Arteriosclerosis enzymology
Arteriosclerosis pathology
Carotid Artery Diseases enzymology
Carotid Artery Diseases pathology
Gene Expression Regulation, Enzymologic genetics
Gene Expression Regulation, Enzymologic immunology
Isoenzymes analysis
Prostaglandin-Endoperoxide Synthases analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1078-5884
- Volume :
- 20
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery
- Publication Type :
- Academic Journal
- Accession number :
- 10942685
- Full Text :
- https://doi.org/10.1053/ejvs.2000.1145