Back to Search Start Over

Quality control of benserazide-levodopa and carbidopa-levodopa tablets by capillary zone electrophoresis.

Authors :
Fanali S
Pucci V
Sabbioni C
Raggi MA
Source :
Electrophoresis [Electrophoresis] 2000 Jul; Vol. 21 (12), pp. 2432-7.
Publication Year :
2000

Abstract

In modern practice, the treatment of Parkinson's disease and syndrome is carried out using pharmaceutical formulations containing a combination of levodopa and a decarboxylation inhibitor (carbidopa or benserazide). Two pharmaceutical formulations were quantified by capillary zone electrophoresis using two procedures which differed only in the kind of background electrolyte used. One procedure used a 25 mM phosphate buffer, pH 2.5, while the second one used a 25 mM borate buffer, pH 8.5. The electrophoretic analysis was carried out using an uncoated fused- silica capillary, a separation voltage of 20 kV with currents typically less than 60 microA, and spectrophotometric detection at 205 nm. Calibration curves were performed for levodopa (concentration range 1-100 microg/mL), for carbidopa and benserazide (1-50 microg/mL), and the plots of the peak area versus concentration were found to be linear with a correlation coefficient better than 0.9990. Satisfactory results were obtained when commercial tablets were analyzed in terms of accuracy (98-102%), repeatability (0.6-2.0%), and intermediate precision (1.1-2.6%).

Details

Language :
English
ISSN :
0173-0835
Volume :
21
Issue :
12
Database :
MEDLINE
Journal :
Electrophoresis
Publication Type :
Academic Journal
Accession number :
10939456
Full Text :
https://doi.org/10.1002/1522-2683(20000701)21:12<2432::AID-ELPS2432>3.0.CO;2-E