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Hypothalamic adrenergic receptor changes in the metabolic syndrome of genetically obese (ob/ob) mice.
- Source :
-
American journal of physiology. Regulatory, integrative and comparative physiology [Am J Physiol Regul Integr Comp Physiol] 2000 Aug; Vol. 279 (2), pp. R505-14. - Publication Year :
- 2000
-
Abstract
- The genetically, seasonally, and diet-induced obese, glucose-intolerant states in rodents, including ob/ob mice, have each been associated with elevated hypothalamic levels of norepinephrine (NE). With the use of quantitative autoradiography on brain slices of 6-wk-old obese (ob/ob) and lean mice, the adrenergic receptor populations in several hypothalamic nuclei were examined. The binding of [(125)I]iodocyanopindolol to beta(1)- and beta(2)-adrenergic receptors in ob/ob mice was significantly increased in the paraventricular hypothalamic nucleus (PVN) by 30 and 38%, in the ventromedial hypothalamus (VMH) by 23 and 72%, and in the lateral hypothalamus (LH) by 10 and 15%, respectively, relative to lean controls. The binding of [(125)I]iodo-4-hydroxyphenyl-ethyl-aminomethyl-tetralone to alpha(1)-adrenergic receptors was also significantly increased in the PVN (26%), VMH (67%), and LH (21%) of ob/ob mice. In contrast, the binding of [(125)I]paraiodoclonidine to alpha(2)-adrenergic receptors in ob/ob mice was significantly decreased in the VMH (38%) and the dorsomedial hypothalamus (17%) relative to lean controls. This decrease was evident in the alpha(2A)- but not the alpha(2BC)-receptor subtype. Scatchard analysis confirmed this decreased density of alpha(2)-receptors in ob/ob mice. Together with earlier studies, these changes in hypothalamic adrenergic receptors support a role for increased hypothalamic NE activity in the development of the metabolic syndrome of ob/ob mice.
- Subjects :
- Animals
Autoradiography
Clonidine analogs & derivatives
Clonidine metabolism
Female
In Vitro Techniques
Iodocyanopindolol metabolism
Mice
Phenethylamines metabolism
Reference Values
Tissue Distribution
Hypothalamus metabolism
Obesity genetics
Obesity metabolism
Receptors, Adrenergic, alpha metabolism
Receptors, Adrenergic, beta metabolism
Tetralones
Subjects
Details
- Language :
- English
- ISSN :
- 0363-6119
- Volume :
- 279
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Regulatory, integrative and comparative physiology
- Publication Type :
- Academic Journal
- Accession number :
- 10938239
- Full Text :
- https://doi.org/10.1152/ajpregu.2000.279.2.R505