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Determination of plasma free fatty acids by high-performance liquid chromatography with electrochemical detection.
- Source :
-
Analytical biochemistry [Anal Biochem] 2000 Aug 15; Vol. 284 (1), pp. 65-9. - Publication Year :
- 2000
-
Abstract
- Determination of free fatty acids (FFAs) in control serum and human plasma was carried out by high-performance liquid chromatography with electrochemical detection. Peak height for palmitic, palmitoleic, stearic, oleic, linoleic, and arachidonic acids at a detection potential of -415 mV vs a saturated calomel electrode showed a linear relation to acid amount in the range 50-1600 pmol. The present method for plasma free fatty acid determination required only 10 microL of plasma sample. The method is simple and the time for blood pretreatment is short. Change in plasma FFA with blood glucose level was monitored before and after meal ingestion by this method, using one male and one female subject. It was possible to rapidly reduce plasma FFA and increase blood glucose subsequent to the meal. The present method is thus shown to have potential for clinical application.<br /> (Copyright 2000 Academic Press.)
- Subjects :
- Adult
Arachidonic Acid blood
Blood Glucose analysis
Fatty Acids, Monounsaturated blood
Female
Humans
Linoleic Acid blood
Male
Middle Aged
Oleic Acids blood
Palmitic Acids blood
Stearic Acids blood
Time Factors
Chromatography, High Pressure Liquid methods
Electrochemistry
Fatty Acids, Nonesterified blood
Subjects
Details
- Language :
- English
- ISSN :
- 0003-2697
- Volume :
- 284
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Analytical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 10933857
- Full Text :
- https://doi.org/10.1006/abio.2000.4682