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High frequencies of circulating melanoma-reactive CD8+ T cells in patients with advanced melanoma.
- Source :
-
International journal of cancer [Int J Cancer] 2000 Sep 01; Vol. 87 (5), pp. 659-64. - Publication Year :
- 2000
-
Abstract
- To determine whether circulating tumor-reactive T cells are present in melanoma patients, unstimulated T cells from peripheral blood were tested for recognition of HLA-A2- or HLA-A1-matched melanoma cell lines using the ELISPOT assay. Eleven out of 19 patients with metastatic melanoma had a T-cell response with up to 0.81%, 0.78%, 0. 53%, 0.12%, 0.10%, 0.09%, 0.07%, 0.06%, 0.06%, 0.04%, and 0.04% of peripheral blood mononuclear cells (PBMC) secreting IFNgamma upon exposure to various HLA-A2- or HLA-A1-matched melanoma cell lines. These T-cell responses were mediated by CD8+ T cells and could specifically be blocked by an anti-HLA-A2 antibody in HLA-A2-positive patients. Separation experiments performed in one melanoma patient showed tumor-reactive T cells in both the CD8+ effector T cell (CD45RA+/IFNgamma+) as well as the CD8+ memory T-cell compartment (CD45RO+/IFNgamma+). In 3 out of 5 patients, in whom autologous cell lines were available, similar frequencies of T cells in response to HLA-A1- or HLA-A2-matched allogeneic and autologous tumor cells were observed, while 2 patients had a T-cell response restricted to either the autologous or the allogeneic cell lines. These results give evidence for the presence of tumor-reactive CD8+ T cells in more than half of melanoma patients tested. Although some of these patients have clinical evidence for an immunological-mediated tumor control, several patients have growing tumors suggesting presence of escape mechanisms.<br /> (Copyright 2000 Wiley-Liss, Inc.)
- Subjects :
- Antigens, Neoplasm immunology
CD8-Positive T-Lymphocytes cytology
CD8-Positive T-Lymphocytes metabolism
Epitopes, T-Lymphocyte immunology
HLA-A1 Antigen immunology
HLA-A2 Antigen immunology
Humans
Interferon-gamma immunology
Interferon-gamma metabolism
Lymphocyte Activation immunology
Melanoma blood
T-Lymphocyte Subsets immunology
T-Lymphocytes, Regulatory cytology
T-Lymphocytes, Regulatory metabolism
Tumor Cells, Cultured immunology
CD8-Positive T-Lymphocytes immunology
Melanoma immunology
T-Lymphocytes, Regulatory immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0020-7136
- Volume :
- 87
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- International journal of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 10925359