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A comparison of mutation spectra detected by the Escherichia coli lac(+) reversion assay and the Salmonella typhimurium his(+) reversion assay.
- Source :
-
Mutagenesis [Mutagenesis] 2000 Jul; Vol. 15 (4), pp. 317-23. - Publication Year :
- 2000
-
Abstract
- Each of the Escherichia coli tester strains in the WP3101P-WP3106P series contains an F' plasmid with a different base substitution mutation within the lacZ gene. Each of the six possible base substitution mutations, therefore, can be assayed with these strains by Lac(+) reversion. We used the strains to characterize the mutational profiles of 21 chemical mutagens, including alkylating agents, base analogs and oxidative compounds. We also assayed the mutagens with Salmonella typhimurium tester strains TA7002, TA7004 and TA7005, which detect A.T-->T.A, G.C-->A.T and G.C-->T.A mutations, respectively, and we compared the sensitivity and specificity of the two systems. Escherichia coli strain WP3102P was more sensitive than the S.TYPHIMURIUM: strains to G.C-->A.T transitions induced by N(4)-aminocytidine, 5-azacytidine, cumene hydroperoxide (CHP), t-butyl hydroperoxide (BHP), N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG), methyl methane sulfonate and N-ethyl-N-nitrosourea (ENU), while the reverse was true for G.C-->A.T transitions induced by 2-aminopurine and phosmet. Escherichia coli strain WP3104P, which detects G.C-->T.A transversions, was superior to the S.TYPHIMURIUM: strains in detecting transversions induced by N(4)-aminocytidine, 5-azacytidine, 5-diazouracil, CHP, BHP, ENNG, ENU, 4-nitroquinoline 1-oxide (4-NQO) and 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX). Escherichia coli WP3105P was also more sensitive than S. TYPHIMURIUM: to A.T-->T.A transversions induced by N-methyl-N- nitrosourea (MNU), CHP and 4-NQO, but it was less sensitive to those induced by ENNG, ENU and 2-aminopurine. The present results indicate that the E.COLI: Lac(+) reversion system with tester strains WP3101P-WP3106P is as sensitive as the S.TYPHIMURIUM: His(+) reversion system for the detection of specific mutations induced by a variety of direct mutagens.
- Subjects :
- 2-Aminopurine metabolism
4-Nitroquinoline-1-oxide metabolism
Alkylating Agents metabolism
Azacitidine metabolism
Benzene Derivatives metabolism
Cytidine analogs & derivatives
Cytidine metabolism
DNA drug effects
Dose-Response Relationship, Drug
Ethylnitrosourea metabolism
Formaldehyde metabolism
Furans metabolism
Furylfuramide metabolism
Glyoxal metabolism
Histidine metabolism
Lac Operon genetics
Methylnitronitrosoguanidine analogs & derivatives
Methylnitronitrosoguanidine metabolism
Mutagenicity Tests methods
Oxidants metabolism
Phosmet metabolism
Plasmids metabolism
Point Mutation drug effects
Sodium Azide metabolism
Uracil analogs & derivatives
Uracil metabolism
tert-Butylhydroperoxide metabolism
DNA Mutational Analysis methods
Escherichia coli genetics
Mutagens
Salmonella typhimurium genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0267-8357
- Volume :
- 15
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Mutagenesis
- Publication Type :
- Academic Journal
- Accession number :
- 10887210
- Full Text :
- https://doi.org/10.1093/mutage/15.4.317