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Distribution of non-collagenous dentin matrix proteins and proteoglycans, and their relation to calcium accumulation in bisphosphonate-affected rat incisors.
- Source :
-
European journal of oral sciences [Eur J Oral Sci] 2000 Jun; Vol. 108 (3), pp. 222-32. - Publication Year :
- 2000
-
Abstract
- It has been reported that multiple injections of 1-hydroxyethylidene- 1,1-bisphosphonate (HEBP) to rats prevent mineralization of incisor dentin, thereby revealing high concentrations of calcium in the non-mineralized matrix of circumpulpal dentin. To identify the molecules responsible for calcium accumulation in circumpulpal dentin matrix, rats were injected daily with HEBP (8 mg P/kg) for 7 d, and the incisors processed for various histochemical and immunohistochemical staining of non-collagenous matrices of dentin. Cuprolinic blue reactions for proteoglycans (PGs) were equally distributed in non-mineralized matrix of mantle and circumpulpal dentin layers. Dentin sialoprotein (DSP) and osteopontin (OPN) immunoreactions were found in non-mineralized circumpulpal dentin matrix, but not in mantle dentin. In normal incisors, however, predentin matrix showing significant DSP immunoreactivity was negative for Ca-GBHA reactions. HEBP-affected, non-mineralized OPN immunopositive bone matrix was also non-reactive for calcium. From these observations, neither PGs, OPN nor DSP appear to be responsible for calcium accumulation in HEBP-affected circumpulpal dentin. Stains-all reactive component, possibly dentin phosphoprotein (DPP), only showed the same distribution as that of Ca-GBHA in both HEBP-affected and normal dentin matrix, implicating a possible contribution of DPP to calcium accumulation in circumpulpal dentin and, hence, to appositional mineralization of dentin.
- Subjects :
- Animals
Dentin drug effects
Extracellular Matrix drug effects
Extracellular Matrix Proteins analysis
Immunohistochemistry
Male
Phosphoproteins analysis
Proteoglycans analysis
Rats
Rats, Wistar
Sialoglycoproteins analysis
Staining and Labeling
Tooth Calcification physiology
Calcium metabolism
Dentin chemistry
Etidronic Acid pharmacology
Extracellular Matrix chemistry
Tooth Calcification drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0909-8836
- Volume :
- 108
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- European journal of oral sciences
- Publication Type :
- Academic Journal
- Accession number :
- 10872993
- Full Text :
- https://doi.org/10.1034/j.1600-0722.2000.108003222.x